Ordering Details
Cat Number:	AP7278a-ev
Size:	50uL
Price:	£117.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

EN1 [Engrailed 1] [N-term] [RB13885], Rabbit Polyclonal Antibody

Partner: Abcepta, Inc.

Applications:	IF, IHC-P, WB
Species Reactivity:	Human, Mouse
Isotype:	Rabbit Ig
Purification:	Affinity Purified Rabbit Polyclonal Antibody
Description:	Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
Further Information:	This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).
Synonym:	Homeobox protein engrailed-1, Homeobox protein en-1, Hu-En-1, EN1
Ascension Number:	Q05925
NCBI EntrezGene:	2019
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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Fluorescent image of U251 cell stained with EN1 Antibody (N-term)(Cat#AP7278a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37?).Cytoplasmic actin was counterstained with Alexa Fluor� 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?).EN1 immunoreactivity is localized to Nucleus significantly.