Anti-CASC3 Antibody (Y181) at 1:1000 dilution + NCI-H460 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDaBlocking/Dilution buffer: 5% NFDM/TBST.