Western blot analysis of lysates from A431, Hela, U-937, BA/F3 cell line (from left to right), using PGK1 Antibody (S320) (Cat. #AP7169a). AP7169a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.