All lanes : Anti-CBS Antibody (Center) at 1:500 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: LNCap whole cell lysateLane 4: mouse liver lysateLane 5: mouse pancreas lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDaBlocking/Dilution buffer: 5% NFDM/TBST.