All lanes : Anti-CTSE Antibody (Center) at 1?500-1?2000 dilutionLane 1: Mouse spleen whole tissue lysateLane 2: MKN45 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1?10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.