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All lanes : Anti-ST6 Antibody (C253) at 1:1000 dilutionLane 1: Jurkat whole cell lysateLane 2: Raji whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.