All lanes : Anti-WNT5A Antibody (Center) at 1:2000 dilutionLane 1: human heart lysateLane 2: PANC-1 whole cell lysateLane 3: mouse heart lysateLane 4: rat heart lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.