Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-251 MG cells labeling USP17L24 with AP5491b at 1/25 dilution, followed by Dylight� 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus staining on U-251 MG cell line. Cytoplasmic actin is detected with Dylight� 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).