Fluorescent confocal image of Hela cell stained with HIF1A Antibody (N-term)(Cat#AP4776a). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIF1A primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37?).Cytoplasmic actin was counterstained with Alexa Fluor� 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?). Nuclei were counterstained with DAPI (blue) (10 �g/ml, 10 min).HIF1A immunoreactivity is localized to cytoplasm and nucleus significantly.
