Contactin-4 / Big-2 Antibody (0.5ug/ml) staining of Mouse Olfactory bulb (Lanes 1 and 3) and Cerebral cortex (Lanes 2 and 4), comparing wildtype (lanes 3 and 4) with KO mice (Lanes 1 and 2). The last lane contains a lysate of HEK293 overexpressing Mouse Cntn4 (lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Gerrald Lodewijk and Peter Burbach, Rudolf Magnus Institute, Utrecht, Netherlands.