HSP31 Antibody (0.05ug/ml) staining of Yeast lysate (wt) and the KO (delta) in the left panel and the Ponceau stain in the right panel (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained by Dr. M. Skoneczny, IBB, Warsaw, Poland.