Anti MBP Antibody (Y203) at 1:16000 dilution + human cerebellum lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.