All lanes : Anti-Collybistin(Arhgef9) (Center) at 1:1000 dilutionLane 1: rat brain lysateLane 2: rat heart lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.