All lanes : Anti-APLP1 Antibody (C-Term) at 1:2000 dilutionLane 1: U-251 MG whole cell lysateLane 2: U-87 MG whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 72 kDaBlocking/Dilution buffer: 5% NFDM/TBST.