All lanes : Anti-MYL2 Antibody (Center) at 1:2000 dilutionLane 1: Human heart lysateLane 2: Human skeletal muslce lysateLane 3: Mouse heart lysateLane 4: Rat heart lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.