All lanes : Anti-CHRNE Antibody (Center) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Mouse skeletal muscle lysateLane 3: Rat skeletal muscle lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.