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All lanes : Anti-STMN1 Antibody (C-Term) at 1:2000 dilutionLane 1: HT-29 whole cell lysateLane 2: U-2OS whole cell lysateLane 3: PC-12 whole cell lysateLane 4: Hela whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.