All lanes : Anti-Vinculin at 1:2000 dilutionLane 1: human skeletal muscle lysateLane 2: A431 whole cell lysateLane 3: Hela whole cell lysateLane 4: mouse kidney lysateLane 5: PC-12 whole cell lysateLane 6: HepG2 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 117 kDaBlocking/Dilution buffer: 5% NFDM/TBST.