All lanes : Anti-Profilin-1 Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: HUVEC whole cell lysateLane 3: Jurkat whole cell lysateLane 4: 293 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: C6 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDaBlocking/Dilution buffer: 5% NFDM/TBST.