All lanes : Anti-beta Tubulin at 1:2000 dilutionLane 1: C2C12 whole cell lysateLane 2: L6 whole cell lysateLane 3: mouse brain lysateLane 4: mouse spleen whole cell lysateLane 5: mouse thymus lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.