All lanes : Anti-PIGC Antibody (Center) at 1:2000 dilutionLane 1: HL-60 whole cell lysateLane 2: K562 whole cell lysateLane 3: MCF-7 whole cell lysateLane 4: MOLT-4 whole cell lysateLane 5: SH-SY5Y whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.