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All lanes : Anti-ARID3A Antibody (N-Term) at 1:8000 dilutionLane 1: HepG2 whole cell lysateLane 2: K562 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63 kDaBlocking/Dilution buffer: 5% NFDM/TBST.