Ordering Details
Cat Number:	AP21888b
Size:	200uL
Price:	£304.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

ATP5E [C-term] [RB53868], Rabbit Polyclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB
Species Reactivity:	Human
Isotype:	Rabbit IgG
Purification:	Purified Rabbit Polyclonal Antibody
Description:	Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits (By similarity).
Further Information:	This ATP5E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from human ATP5E.
Synonym:	ATP synthase subunit epsilon, mitochondrial, ATPase subunit epsilon, ATP5E
Ascension Number:	P56381
NCBI EntrezGene:	514
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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All lanes : Anti-ATP5E Antibody (C-Term) at 1:2000 dilutionLane 1: human heart lysateLane 2: human skeletal muscle lysateLane 3: Hela whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 6 kDaBlocking/Dilution buffer: 5% NFDM/TBST.