All lanes : Anti-Zebrafish wnt8a Antibody (Center) at 1:1000-1:2000 dilutionLane 1: zebrafish muscle lysateLane 2: ZF4 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.