All lanes : Anti-srrt Antibody (C-Term) at 1:2000 dilutionLane 1: Zebrafish muscle lysatesLane 2: Zebrafish lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 103 kDaBlocking/Dilution buffer: 5% NFDM/TBST.