All lanes : Anti-VDAC2 Antibody (N-term) at 1:4000 dilutionLane 1: NCI-H1299 whole cell lysateLane 2: HCT116 whole cell lysateLane 3: PC-3 whole cell lysateLane 4: Mouse heart tissue lysateLane 5: Rat heart tissue lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.