All lanes : Anti-GSTT1 Antibody (C-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.