All lanes : Anti-ACBD4 Antibody (Center) at 1:2000 dilutionLane 1: T47D whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: HT-1080 whole cell lysatesLane 4: human liver lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.