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All lanes : Anti-AATK Antibody (N-Term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: NIH/3T3 whole cell lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 145 kDaBlocking/Dilution buffer: 5% NFDM/TBST.