All lanes : Anti-becn1 Antibody (C-Term) at 1:2000 dilutionLane 1: Zebrafish lysatesLane 2: ZF4 whole cell lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.