Anti-gabarapa Antibody (N-Term)at 1:500 dilution + zebrafish lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 14 kDaBlocking/Dilution buffer: 5% NFDM/TBST.