All lanes : Anti-CHST1 Antibody (C-term) at 1:2000 dilutionLane 1: human brain lysatesLane 2: Hela whole cell lysatesLane 3: Jurkat whole cell lysatesLane 4: mouse brain lysatesLane 5: rat brain lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.