All lanes : Anti-NPY2R Antibody (N-term) at 1:2000 dilutionLane 1: mouse brain lysatesLane 2: rat brain lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.