All lanes : Anti-EHD2 Antibody (C-term) at 1:1000 dilutionLane 1: Human heart tissue lysateLane 2: A549 whole cell lysateLane 3: Human placenta tissuee lysateLane 4: Hela whole cell lysateLane 5: Mouse kidney tissue lysateLane 6: Rat lung tissue lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDaBlocking/Dilution buffer: 5% NFDM/TBST.