All lanes : Anti-WDR82 Antibody (N-term) at 1:2000 dilutionLane 1: NCCIT whole cell lysateLane 2: SH-SY5Y whole cell lysateLane 3: HCT116 whole cell lysateLane 4: U-251 MG whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDaBlocking/Dilution buffer: 5% NFDM/TBST.