All lanes : Anti-WDR82 Antibody (N-term) at 1:1000 dilutionLane 1: F9 whole cell lysateLane 2:NIH/3T3 whole cell lysateLane 3: SH-SY5Y whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDaBlocking/Dilution buffer: 5% NFDM/TBST.