Western blot analysis of lysates from zebrafish brain, Zebrafish tissue lysate (from left to right), using (DANRE) gfap Antibody (N-term)(Cat. #AP20618a). AP20618a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.