All lanes : Anti-ANKS1A Antibody (N-term) at 1:2000 dilutionLane 1: HCT116 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 123 kDaBlocking/Dilution buffer: 5% NFDM/TBST.