All lanes : Anti-FCGRT Antibody (C-term) at 1:500 dilutionLane 1: HepG2 whole cell lysateLane 2: THP-1 whole cell lysateLane 3: Human lung lysateLane 4: COLO 205 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.