Ordering Details
Cat Number:	AP1821a-ev
Size:	80uL
Price:	£117.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

GABARAP [N-term] [RB11846], Rabbit Polyclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB, IHC-P, IF
Species Reactivity:	Human, Mouse, Rat
Isotype:	Rabbit IgG
Purification:	Affinity Purified Rabbit Polyclonal Antibody
Description:	Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
Further Information:	This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids of human GABARAP.
Synonym:	Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, MM46, GABARAP, FLC3B
Ascension Number:	O95166
NCBI EntrezGene:	11337
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line)(Hela-C:Serum-starve overnight;Hela-chloroquine?50 ?M, overnight; right) cells labeling GABARAP with AP1821a at 1/25 dilution, followed by Dylight� 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and autophagic vacuoles staining on HeLa cell line. Cytoplasmic actin is detected with Dylight� 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).