Anti-CALCRL Antibody (Center) at 1:2000 dilution + Human heart lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.