Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling LZIC with AP17735 at 1/25 dilution, followed by Dylight� 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus and Cytoplasm?Weak Membrane staining on Hela cell line. The nuclear counter stain is DAPI (blue).