All lanes : Anti-FUBI Antibody (C-term) at 1:2000 dilutionLane 1: HL-60 whole cell lysateLane 2: Jurkat whole cell lysateLane 3: MCF-7 whole cell lysateLane 4: PC-3 whole cell lysateLane 5: rat cerebellum lysateLane 6: mouse liver lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 7 kDaBlocking/Dilution buffer: 5% NFDM/TBST.