All lanes : Anti-hCalmodulin-D132 at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: SH-SY5Y whole cell lysateLane 4: NIH/3T3 whole cell lysateLane 5: Rat brain lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.