Ordering Details
Cat Number:	AP1480c-ev
Size:	80uL
Price:	£117.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

Alkaline Phosphatase [ALPI] [Centre] [RB13411], Rabbit Polyclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB, IHC-P, IF
Species Reactivity:	Human
Isotype:	Rabbit IgG
Purification:	Purified Rabbit Polyclonal Antibody
Description:	There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Further Information:	This Alkaline Phosphatase (ALPI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human Alkaline Phosphatase (ALPI).
Synonym:	Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI
Ascension Number:	P09923
NCBI EntrezGene:	248
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) (Center) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1480c Alkaline Phosphatase (ALPI) (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). Alkaline Phosphatase (ALPI) immunoreactivity is localized to the cytoplasm of HeLa cells.