(LEFT)Western blot analysis of anti-EphA3 Pab in CHO cell lysate. EphA3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of EphA3 (arrow) using rabbit polyclonal EphA3 Antibody (C-term). 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA3 gene (Lane 2)