All lanes : Anti-PDCD4 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: Jurkat whole cell lysateLane 3: HepG2 whole cell lysateLane 4: 293 whole cell lysateLane 5: HL-60 whole cell lysateLane 6: Mouse thymus tissue lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.