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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 cells labeling Csf1r with AP13911B at 1/25 dilution, followed by Dylight� 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm staining on NIH/3T3 cell line. Cytoplasmic actin is detected with Dylight� 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).