All lanes : Anti-VSGP/F-spondin Antibody (N-term) at 1:2000 dilutionLane 1: Human heart lysateLane 2: Human lung lysateLane 3: Mouse lung lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 91 kDaBlocking/Dilution buffer: 5% NFDM/TBST.