All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: HepG2 whole cell lysateLane 3: MDA-MB-231 whole cell lysateLane 4: K562 whole cell lysateLane 5: Mouse brain tissue lysateLane 6: Mouse cerebellum tissue lysateLane 7: PC-12 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.